This study was aimed to develop and validate the difference and visible spectrophotometric methods for the quantitative estimation of esomeprazole in pure form and formulations. In difference spectrophotometric method 0.1N HCl and 0.1N NaOH were used as solvents to induce the spectral changes in order to measure the difference in the absorbance. The difference absorbance maximum was observed at 307 nm. Beer-Lambert’s law was obeyed at a concentration range of 5-25 μg/ml with a limit of detection and limit of quantification of 0.872 μg/ml and 2.64 μg/ml respectively. The coefficient of correlation (r2) was found to be 0.998. A visible spectrophotometric method was developed by using 0.05% w/v bromocresol green solution as chromogenic agent. Linearity was found at a concentration range of 5-25 μg/ml at max of 691 nm. The coefficient of correlation (r2) was observed as 0.997. The developed methods were validated as per ICH Q2 (R1) guidelines and all the results obtained were in accordance with those of the official limits. The proposed methods were successfully applied for the analysis of esomeprazole tablets (NEXPRO-40 mg) and the assay values were 100.62% and 99.89% respectively. The difference spectrophotometric method found to be more sensitive than visible spectrophotometric method. Both the methods can be applied for routine quality control analysis of esomeprazole.
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